Conjugation
OriTF

Part:BBa_J01002:Design

Designed by: Golden Bear   Group: iGEM2005   (2005-10-18)


OriT-F (Origin of Transfer for the F-plasmid nic region)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 234
    Illegal NheI site found at 280
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Sequence for this part is obtained from:
GenBank Accession #:AP001918, BCT 17-NOV-2005 [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=8918823&itemID=192&view=gbwithparts link]

References

Thompson, R., Taylor, L., Kelly, K., Everett, R., and Willetts, N. (1984) "The F plasmid origin of transfer: DNA sequence of wild-type and mutant origins and location of origin-specific nicks." EMBO J. 3, 1175–1180. [http://www.pubmedcentral.gov/articlerender.fcgi?tool=pubmed&pubmedid=6329741 link]

Frost, L.S., Ippen-Ihler, K. and Skurray, R.A. (1994) "Analysis of the sequence and gene products of the transfer region of the F sex factor," Microbiol. Rev. 58 (2), 162-210. PUBMED 7915817 [http://mmbr.asm.org/cgi/reprint/58/2/162?view=long&pmid=7915817 link]